We have developed an online database describing the known cell lines from Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera that originated from crop pest insects. Cell line information has been primarily obtained from previous compilations of insect cell lines. We conducted in-depth internet literature searches and drew on internet sources such as the Cellosaurus database, and inventories from cell line depositories. Here, we report on a new database of insect cell lines, which covers >700 cell lines from >80 species. We provide the designation, tissue and species of origin, cell line developer, unique characteristics, its use in various applications, publications, and patents, and, when known, insect virus susceptibility. This database will be updated on an ongoing basis. Please send updates to Dr. Surjeet Kumar Arya.