Bayer/BCIRL-SfNS2-0813-TR
Order
Species
Common Name(s)
Fall armyworm
Primary Designation
Bayer/BCIRL-SfNS2-0813-TR
Stage
Tissue
Initiator(s)
T. Reall
References
Reall, T., Kraus, S., Goodman, C. L., Ringbauer, J., Geibel, S., & Stanley, D. (2019). Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro Cellular & Developmental Biology-Animal, 55(9), 686-693.
Notes
Growth characteristics: population doubling time = 46.4 hr; cell mean diameter: 13.7 µm; Authentication:PCR analysis of genomic DNA from cell line resulted in amplicon patterns similar to other Sf tissues; Microbial presence: cell line tested negative for the presence of mycoplasma.
Medium
TR#14 (1/4 L-15 + 1/4 EX-CELL 420 + 1/2 TNM-FH) or CLG#2 (1/2 L15 + 1/2 EX-CELL 420) + 10%FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
david.stanley@usda.gov
