Bayer/BCIRL-SfNS3-0827-TRL

Submitted by Anonymous (not verified) on March 9, 2022 - 11:20am
Order: 
Lepidoptera
Species: 
Spodoptera frugiperda
Common Name(s): 
Fall armyworm
Primary Designation: 
Bayer/BCIRL-SfNS3-0827-TRL
Accession No.: 
CVCL_XA66
Other Designations: 
SfNS3-0827
Stage: 
Larva
Tissue: 
Central Nervous System
Initiator(s): 
T. Reall
References: 
Reall, T., Kraus, S., Goodman, C. L., Ringbauer, J., Geibel, S., & Stanley, D. (2019). Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro Cellular & Developmental Biology-Animal, 55(9), 686-693.
Notes: 
Growth characteristics: population doubling time = 141.4 hr; cell mean diameter: 11.3 µm; Authentication: PCR analysis of genomic DNA from cell line resulted in amplicon patterns similar to other Sf tissues; Microbial presence: cell line tested negative for the presence of mycoplasma.
Medium: 
TR#12 (TNM-FH + 1/2NQ(GS21)) or CLG#2 (1/2 L15 + 1/2 EX-CELL 420) + 10% FBS
Cell Culture Collection: 
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
Status: 
Available