Bayer/BCIRL-SfNS4-0827-TR
Order
Species
Common Name(s)
Fall armyworm
Primary Designation
Bayer/BCIRL-SfNS4-0827-TR
Accession No.
CVCL_XA67
Stage
Tissue
Initiator(s)
T. Reall
References
Reall, T., Kraus, S., Goodman, C. L., Ringbauer, J., Geibel, S., & Stanley, D. (2019). Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro Cellular & Developmental Biology-Animal, 55(9), 686-693.
Notes
Growth characteristic = population doubling time: 127.8 hr; cell mean diameter: 13.1 µm; Authentication: PCR analysis of genomic DNA from cell line resulted in amplicon patterns similar to other Sf tissues; Microbial presence: cell line tested negative for the presence of mycoplasma.
Medium
TR#13 (1/2 TnM-FH + 1/4 (NeuralQ + 1/2 GS21) + 1/4 H2O) or TR#14 (1/4 L-15 + 1/4 EX-CELL 420 + 1/2 TnM-FH) + 10%FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
david.stanley@usda.gov
