BCIRL-HaAM1
Order
Species
Common Name(s)
cotton bollworm
Primary Designation
BCIRL-HaAM1
Accession No.
CVCL_Z402
Stage
Tissue
Initiator(s)
A. H. McIntosh
References
McIntosh, A. H., Ignoffo, C. M., Quhou, C., & Pappas, M. (1983). Establishment of a cell line from Heliothis armigera (HBN.)(Lepidoptera: Noctuidae). In Vitro, 19(8), 589-590; McIntosh, A. H., Grasela, J. J., & Matteri, R. L. (1996). Identification o finsect cell lines by DNA amplification fingerprinting (DAF). Insect Molecular Biology, 5(3), 187-195.
Notes
Morphology: most cells are oval and round; Karyology: chromosomes is typical of Lepidoptera (i.e., short condensed rods and spheres; a majority of cells are polyploid); Growth characteristics: the cell line is split (1:2) every 1 or 2 wk.
Medium
EX-CELL 420 + 10% FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
david.stanley@usda.gov
Status
Microorganism Propagation
Unable to replicate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV), Helicoverpa armigera or Autographa californica multiple nucleopolyhedrovirus (HearMNPV, AcMNPV)
