BCIRL-HV-AM1
Order
Species
Common Name(s)
Tobacco budworm
Primary Designation
BCIRL-HV-AM1
Accession No.
CVCL_Z397
Stage
Tissue
Initiator(s)
A. H. McIntosh
References
McIntosh, A. H., & PA, A. (1981). ESTABLISHMENT OF TWO CONTINUOUS CELL LINES OF HELIOTHIS VIRESCENS (F.)(LEPIDOPTERA: NOCTUIDAE); McIntosh, A. H., & Ignoffo, C. M. (1981). REPLICATION AND INFECTIVITY OF THE SINGLE-EMBEDDED NUCLEAR POLYHEDROSIS VIRUS, BACULOVIRUS HELIOTHIS, IN HOMOLOGOUS CELL LINES. Journal of Invertebrate Pathology, 37(3), 258-264; McIntosh, A. H., & Ignoffo, C. M. (1983). CHARACTERIZATION OF FIVE CELL LINES ESTABLISHED FROM SPECIES OF HELIOTHIS. Applied Entomology and Zoology, 18(2), 262-269; McIntosh, A. H., & Ignoffo, C. M. (1989). REPLICATION OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS IN FIVE LEPIDOPTERAN CELL LINES. Journal of Invertebrate Pathology, 54(1), 97-102.
Notes
Morphology: this cell line consists of a mixture of epithelial-like and fibroblastlike cells, as well as small, rounded cells; Growth characteristics: maximum cell population per milliliter and doubling time for were 2 x 10e6 cells/ml and 37 h, these cells grow firmly attached to the surface; Authentication: used isoenzyme analysis to distinguish from other lepidopteran cell lines; Microbial presence: mycoplasma-free; Karyology: this cell lines are typical of those described for lepidopteran; cell for most of the apart from diploid to polyploid; 85% of this cell line consists of 60 to 120 chromosome/cell.
Medium
EX-CELL 401 + 10% FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
david.stanley@usda.gov
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and low levels of Helicoverpa zea single nucleopolyhedrovirus (HzSNPV)
