BCIRL-HZ-AM3

BCIRL-HZ-AM3

Order

Species

Common Name(s)

cotton bollworm

Primary Designation

BCIRL-HZ-AM3

Accession No.

CVCL_Z401

Stage

Tissue

Initiator(s)

A.H. McIntosh

References

McIntosh, A. H., & Ignoffo, C. M. (1981). REPLICATION AND INFECTIVITY OF THE SINGLE-EMBEDDED NUCLEAR POLYHEDROSIS VIRUS, BACULOVIRUS HELIOTHIS, IN HOMOLOGOUS CELL LINES. Journal of Invertebrate Pathology, 37(3), 258-264; McIntosh, A. H., & Ignoffo, C. M. (1983). CHARACTERIZATION OF FIVE CELL LINES ESTABLISHED FROM SPECIES OF HELIOTHIS. Applied Entomology and Zoology, 18(2), 262-269.

Notes

Morphology: shape from subspherical to elipsoid; Growth characteristics: Maximum cell population of H. zea-derived lines ranged from 9.97±0.32×10X5 to 12.00±0.44×10X5 cells; Cell doubling time: 31 h; Karyology: more than 90% of the cells contained 40 to 180 chromosomes/cell.

Medium

TC 199-MK medium + 10% FCS

Cell Culture Collection

Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.

Status

Microorganism Propagation

Able to replcate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV).

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu