BCIRL-PX2-HNU3
Order
Species
Common Name(s)
Diamondback moth
Primary Designation
BCIRL-PX2-HNU3
Accession No.
CVCL_Z570
Stage
Tissue
Initiator(s)
Q. Chen
References
Chen, Q., McIntosh, A. H., & Ignoffo, M. (1983). Establishment of a new cell line from the pupae of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Journal of Central China Normal University (Natural Sciences),17, 99-104; McIntosh, A. H., & Ignoffo, C. M. (1989). Replication of Autographa californica nuclear polyhedrosis virus in five lepidopteran cell lines. Journal of Invertebrate Pathology, 54(1), 97-102; McIntosh, A. H. (1991). In vitro infectivity of a clonal isolate of Syngrapha falcifera (celery looper) multiple polyhedrosis virus. Journal of Invertebrate Pathology, 57(3), 441-442; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Li, L., & Chen, Q. (1995). STORAGE OF INSECT CELL LINES BY A SIMPLE METHOD. Journal of Central China Normal University. Natural sciences edition, 29(4), 511-512; McIntosh, A. H., Grasela, J. J., & Matteri, R. L. (1996). Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Molecular Biology, 5(3), 187-195; Alhag, A. K. N., Chao, Y. H., & Xin, P. J. (2007). Identification of insect cell lines from 8 lepidpteran species by DNA amplification fingerprinting. J. Appl. Sci, 7, 4040-4043.
Notes
Morphology: most cells of this line were round and oval in shape, some were elongated or spindle shaped, although there were some other types of cells in the mixed cell population; Growth characteristics: population doubling time at 28 Celsius in 96 hours was 30 ± 2.06 and the cell density reached, from 1x10^5 cells/ml inoculated, a level of approximately 1.3x10^6 cells/ml after 8 days; Karyology: chromosomes are typical of other lepidopteran cells; Authentication: distinguished from cell lines derived from other insect species by isoelectric focusing using the enzyme phosphoglucoisomerase; Unique properties: three recombinant proteins (pseudorabies virus glycoprotein (gp50T), human plasminogen (HPg), and β-galactosidase β-gal) is expressed using baculovirus expresssion system.
Medium
TC-199-MK or EX-CELL 420 + 10% FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
david.stanley@usda.gov
Status
Microorganism Propagation
Replicates Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Syngrupha fulcifera multiple nucleopolyhedrovirus (SfaMNPV) and Plutella xylostella granulosis virus (PxGV)
