Bme21
Order
Species
Common Name(s)
Silk moth
Primary Designation
Bme21
Accession No.
CVCL_Z087
Stage
Tissue
Initiator(s)
T. Kusakabe
References
Kusakabe, T., Aoki, C., Ninagi, O., Lee, J. M., Iiyama, K., & Kawakami, N. (2011). U.S. Patent No. 7,955,793. Washington, DC: U.S. Patent and Trademark Office; Lee, J.M., Kawakami, N., Mon, H., Mitsunobu, H., Iiyama, K., Ninaki, S., Maenaka, K., Park, E.Y. and Kusakabe, T. (2012). Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain. Biotechnology letters, 34(10), pp.1773-1779.
Notes
Morphology: the shape of the cells are small and round with clear cytoplasm measuring about 10–20 µm in diameter; Growth characteristics: doubling time = 48–96 h; Authentication: DNA fingerprinting profiles were created using simple-sequence repeat polymerase chain reaction (SSR-PCR) with four sets of SSR primers exhibited clear differences between the Bme21 cell line and the three B. mori (BmN4, Bm5, and Bmc140) cell lines.
Medium
IPL-41 + 10 % FBS
Cell Culture Collection
International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM P-20852.
Status
Microorganism Propagation
Bombyx mori nucleopolyhedrovirus (BmNPV)
Patents
Kusakabe T. et al. (2011). US Patent No. 7955793
