BmN

BmN

Order

Species

Common Name(s)

Silkworm

Primary Designation

BmN

Accession No.

CVCL_Z633

Tissue

Initiator(s)

T.D.C. Grace

References

Grace, T. D. C. (1967). Establishment of a line of cells from the silkworm Bombyx mori. Nature, 216(5115), 613-613; Volkman, L. E., & Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Applied and Environmental Microbiology, 44(1), 227-233; Maeda, S. (1984). A plaque assay and cloning of Bombyx mori nuclear polyhedrosis virus. The Journal of Sericultural Science of Japan, 53(6), 547-548; Maeda, S., Kawai, T., Obinata, M., Fujiwara, H., Horiuchi, T., Saeki, Y., ... & Furusawa, M. (1985). Production of human α-interferon in silkworm using a baculovirus vector. Nature, 315(6020), 592-594; Maeda, S. (1989) Gene transfer vectors of a baculovirus, Bombyx mori nuclear polyhedrosis virus, and their use for expression of foreign genes in insect cells. In: Invertebrate Cell System Applications (Editor: J. Mitsuhashi), pp. 167-181, CRC Press, Inc. Boca Raton, FL; Ninaki, O., Takada, N., Fujiwara, H., Ogura, T., Miyajima, N., Kiyota, A., & Maekawa, H. (1989). Gene analysis by blot hybridization on the silkworm, Bombyx mori, cell lines; McIntosh, A. H., Grasela, J. J., & Matted, R. L. (1996). Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Molecular Biology, 5(3), 187-195;Grasela, J. J., Mcintosh, A. H., Goodman, C. L., Wilson, L. E., & King, L. A. (2000). Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 36(3), 205-210; Mcintosh, A. H., Grasela, J. J., & Popham, H. J. (2005). AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda. In Vitro Cellular & Developmental Biology-Animal, 41(8-9), 298-304; Yao, H. P., Chen, L., Xiang, X., Guo, A. Q., Lu, X. M., & Wu, X. F. (2010). Proteomics identification and annotation of proteins of a cell line of Bombyx mori, BmN cells. Bioscience Reports, 30(3), 209-215; Geisler, C., & Jarvis, D. L. (2018). Adventitious viruses in insect cell lines used for recombinant protein expression. Protein expression and purification, 144, 25-32.

Notes

Unique properties: used for recombinant protein production; proteome analysis was performed; some proteins related to virus infection were also identified.

Medium

EX-CELL 420 + 10% FBS

Status

Microorganism Propagation

Bombyx mori nucleopolyhedrovirus (BmNPV)

Adventitious Microorganism

Bombyx mori macula-like virus (BmMLV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu