BmN4
Order
Species
Common Name(s)
Silk moth
Primary Designation
BmN4
Accession No.
CVCL_Z634
Tissue
Initiator(s)
T.D.C. Grace
References
Volkman, L. E., Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro (1982) Applied and Environmental Microbiology, 44 (1), 227-223; Yanase, T., Yasunaga, C., & Kawarabata, T. (1998). Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines. Acta Virologica, 42(5), 293-298; Maeda, S. (1989) Gene transfer vectors of a baculovirus, Bombyx mori nuclear polyhedrosis virus, and their use for expression of foreign genes in insect cells. In: Invertebrate Cell System Applications (Editor: J. Mitsuhashi), pp. 167-181, CRC Press, Inc. Boca Raton, FL; Keith, M. B., Farrell, P. J., Iatrou, K., & Behie, L. A. (1999). Screening of transformed insect cell lines for recombinant protein production. Biotechnology Progress, 15(6), 1046-1052; Katsuma, S., Tanaka, S., Omuro, N., Takabuchi, L., Daimon, T., Imanishi, S., ... & Kobayashi, M. (2005). Novel macula-like virus identified in Bombyx mori cultured cells. Journal of virology, 79(9), 5577-5584; Taniai, K., Lee, J. H., & Lee, I. H. (2006). Bombyx mori cell line as a model of immune‐system organs. Insect molecular biology, 15(3), 269-279; Lee, J.M., Kawakami, N., Mon, H., Mitsunobu, H., Iiyama, K., Ninaki, S., Maenaka, K., Park, E.Y. and Kusakabe, T. (2012). Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain. Biotechnology letters, 34(10), pp.1773-1779; Iwanaga, M., Adachi, Y., Uchiyama, K., Tsukui, K., Katsuma, S., & Kawasaki, H. (2014). Long-term adaptation of the Bombyx mori BmN4 cell line to grow in serum-free culture. In Vitro Cellular & Developmental Biology-Animal, 50(9), 792-796; Levin, M., Scheibe, M., & Butter, F. (2020). Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line.
Notes
Morphology (of early passage cultures): contain a number of large irregularly shaped cells with numerous small cytoplasmic processes projecting from their surfaces; the most common cell type is spindle-shaped and 50 to 701 long by 12 to 25 um wide and these cells remain in suspension; the other main cell type is slightly spindle-shaped to round and 18 to 301 um in diameter and these cells attach to the flask surface.
Medium
TC-100 + 10% FBS
Cell Culture Collection
ATCC, CRL-8910; Dr Shigeo Imanishi and Dr Hiroaki Noda of the National Institute of Agrobiological Sciences (Japan)
Status
Microorganism Propagation
Spodoptera exigua multinucleocapsid nucleopolyhedrovirus (SeMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV)
Adventitious Microorganism
Bombyx mori macula-like virus (BmMLV)
