BRL-AG-4
Order
Species
Common Name(s)
boll weevil
Primary Designation
BRL-AG-4
Accession No.
CVCL_Z341
Stage
Tissue
Initiator(s)
B. Stiles
References
Stiles, B., McDonald, I. C., Gerst, J. W., Adams, T. S., & Newman, S. M. (1992). INITIATION AND CHARACTERIZATION OF FIVE EMBRYONIC CELL LINES FROM THE COTTON BOLL WEEVIL ANTHONOMUS GRANDIS IN A COMMERCIAL SERUM-FREE MEDIUM. In Vitro Cellular & Developmental Biology-Animal, 28(5), 355.
Notes
Morphology: elongate and fibroblastoid in appearance, grows as attached cells; Growth characteristics: population doubling time = 20 to 22 hr; Unqiue properties: induced to produce an anti-bacterial factor, anti-bacterial activity; Karyology: exhibited a modal aneuploid chromosome number of 36; Microbial presence: tested negative for mycoplasma contamination using the fluorescent dye Hoechst.
Medium
EX-CELL 420 + 10% FBS
