BTI-Tn-5B1-4
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
BTI-Tn-5B1-4
Accession No.
CVCL_C190
Stage
Tissue
Initiator(s)
R. R. Granados
References
Davis, T. R., Wickham, T. J., McKenna, K. A., Granados, R. R., Shuler, M. L., & Wood, H. A. (1993). COMPARATIVE RECOMBINANT PROTEIN PRODUCTION OF EIGHT INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 29(5), 388-390; Granados, R. R., Guoxun, L., Derksen, A. C., & McKenna, K. A. (1994). A NEW INSECT CELL LINE FROM TRICHOPLUSIA NI (BTI-TN-5B1-4) SUSCEPTIBLE TO TRICHOPLUSIA NI SINGLE ENVELOPED NUCLEAR POLYHEDROSIS VIRUS. Journal of Invertebrate Pathology, 64(3), 260-266; Yanase, T., Yasunaga, C., & Kawarabata, T. (1998). REPLICATION OF SPODOPTERA EXIGUA NUCLEOPOLYHEDROVIRUS IN PERMISSIVE AND NON-PERMISSIVE LEPIDOPTERAN CELL LINES. Acta Virologica, 42(5), 293-298; Saarinen, M. A., Troutner, K. A., Gladden, S. G., Mitchell‐Logean, C. M., & Murhammer, D. W. (1999). RECOMBINANT PROTEIN SYNTHESIS IN TRICHOPLUSIA NI BTI‐TN‐5B1‐4 INSECT CELL AGGREGATES. Biotechnology and bioengineering, 63(5), 612-617; Li, T. C., Scotti, P. D., Miyamura, T., & Takeda, N. (2007). LATENT INFECTION OF A NEW ALPHANODAVIRUS IN AN INSECT CELL LINE. Journal of Virology, 81(20), 10890-10896; Karger, A., Bettin, B., Lenk, M., & Mettenleiter, T. C. (2010). RAPID CHARACTERISATION OF CELL CULTURES BY MATRIX-ASSISTED LASER DESORPTION/IONISATION MASS SPECTROMETRIC TYPING. Journal of virological methods, 164(1-2), 116-121; Wu, Y. L., Jiang, L., Hashimoto, Y., Granados, R. R., & Li, G. X. (2011). ESTABLISHMENT, GROWTH KINETICS, AND SUSCEPTIBILITY TO ACMNPV OF HEAT TOLERANT LEPIDOPTERAN CELL LINES. Virologica Sinica, 26(3), 198-205; Wilde, M., Klausberger, M., Palmberger, D., Ernst, W., & Grabherr, R. (2014). TNAO38, HIGH FIVE AND SF9—EVALUATION OF HOST–VIRUS INTERACTIONS IN THREE DIFFERENT INSECT CELL LINES: BACULOVIRUS PRODUCTION AND RECOMBINANT PROTEIN EXPRESSION. Biotechnology letters, 36(4), 743-749; Geisler, C., & Jarvis, D. L. (2018). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein expression and purification, 144, 25-32; Fu, Y., Yang, Y., Zhang, H., Farley, G., Wang, J., Quarles, K.A., Weng, Z. and Zamore, P.D. (2018). THE GENOME OF THE HI5 GERM CELL LINE FROM TRICHOPLUSIA NI, AN AGRICULTURAL PEST AND NOVEL MODEL FOR SMALL RNA BIOLOGY. Elife, 7, p.e31628; Chen, W., Yang, X., Tetreau, G., Song, X., Coutu, C., Hegedus, D., Blissard, G., Fei, Z. and Wang, P. (2019). A HIGH‐QUALITY CHROMOSOME‐LEVEL GENOME ASSEMBLY OF A GENERALIST HERBIVORE, TRICHOPLUSIA NI. Molecular Ecology Resources, 19(2), pp.485-496.
Notes
Growth characteristics: doubling times = 26 hours; or 34 +/- 1 hours (in static culture), 27 +/- 2 hours (in suspension culture); Unique properties: commonly used for the expression of recombinant proteins with the baculovirus expression vector system (BEVS); Authentication: this cell line was characterized using matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry.
Medium
90% Grace's + 10% FBS
Cell Culture Collection
Available from Thermo Fisher Scientific (Product#B85502)/or Kerafast (Product#ENH127-FP)
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV), Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), Thysanoplusia orichalcea nucleopolyhedrovirus (ThorNPV)
Adventitious Microorganism
Infected with the alphanodavirus TnCLV (Trichoplusi ni cell line virus)
Patents
US Patent No. 7179648, Auhtor: Granados, R. R., & Li, G., Date: 20-February-2007; US Patent No. 5300435, Auhtor: Granados, R. R., Date: 25-September-2013
