Common Name(s)

Cabbage looper

Primary Designation


Accession No.





R. R. Granados


Davis, T. R., Wickham, T. J., McKenna, K. A., Granados, R. R., Shuler, M. L., & Wood, H. A. (1993). COMPARATIVE RECOMBINANT PROTEIN PRODUCTION OF EIGHT INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 29(5), 388-390; Granados, R. R., Guoxun, L., Derksen, A. C., & McKenna, K. A. (1994). A NEW INSECT CELL LINE FROM TRICHOPLUSIA NI (BTI-TN-5B1-4) SUSCEPTIBLE TO TRICHOPLUSIA NI SINGLE ENVELOPED NUCLEAR POLYHEDROSIS VIRUS. Journal of Invertebrate Pathology, 64(3), 260-266; Yanase, T., Yasunaga, C., & Kawarabata, T. (1998). REPLICATION OF SPODOPTERA EXIGUA NUCLEOPOLYHEDROVIRUS IN PERMISSIVE AND NON-PERMISSIVE LEPIDOPTERAN CELL LINES. Acta Virologica, 42(5), 293-298; Saarinen, M. A., Troutner, K. A., Gladden, S. G., Mitchell‐Logean, C. M., & Murhammer, D. W. (1999). RECOMBINANT PROTEIN SYNTHESIS IN TRICHOPLUSIA NI BTI‐TN‐5B1‐4 INSECT CELL AGGREGATES. Biotechnology and bioengineering, 63(5), 612-617; Li, T. C., Scotti, P. D., Miyamura, T., & Takeda, N. (2007). LATENT INFECTION OF A NEW ALPHANODAVIRUS IN AN INSECT CELL LINE. Journal of Virology, 81(20), 10890-10896; Karger, A., Bettin, B., Lenk, M., & Mettenleiter, T. C. (2010). RAPID CHARACTERISATION OF CELL CULTURES BY MATRIX-ASSISTED LASER DESORPTION/IONISATION MASS SPECTROMETRIC TYPING. Journal of virological methods, 164(1-2), 116-121; Wu, Y. L., Jiang, L., Hashimoto, Y., Granados, R. R., & Li, G. X. (2011). ESTABLISHMENT, GROWTH KINETICS, AND SUSCEPTIBILITY TO ACMNPV OF HEAT TOLERANT LEPIDOPTERAN CELL LINES. Virologica Sinica, 26(3), 198-205; Wilde, M., Klausberger, M., Palmberger, D., Ernst, W., & Grabherr, R. (2014). TNAO38, HIGH FIVE AND SF9—EVALUATION OF HOST–VIRUS INTERACTIONS IN THREE DIFFERENT INSECT CELL LINES: BACULOVIRUS PRODUCTION AND RECOMBINANT PROTEIN EXPRESSION. Biotechnology letters, 36(4), 743-749; Geisler, C., & Jarvis, D. L. (2018). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein expression and purification, 144, 25-32; Fu, Y., Yang, Y., Zhang, H., Farley, G., Wang, J., Quarles, K.A., Weng, Z. and Zamore, P.D. (2018). THE GENOME OF THE HI5 GERM CELL LINE FROM TRICHOPLUSIA NI, AN AGRICULTURAL PEST AND NOVEL MODEL FOR SMALL RNA BIOLOGY. Elife, 7, p.e31628; Chen, W., Yang, X., Tetreau, G., Song, X., Coutu, C., Hegedus, D., Blissard, G., Fei, Z. and Wang, P. (2019). A HIGH‐QUALITY CHROMOSOME‐LEVEL GENOME ASSEMBLY OF A GENERALIST HERBIVORE, TRICHOPLUSIA NI. Molecular Ecology Resources, 19(2), pp.485-496.


Growth characteristics: doubling times = 26 hours; or 34 +/- 1 hours (in static culture), 27 +/- 2 hours (in suspension culture); Unique properties: commonly used for the expression of recombinant proteins with the baculovirus expression vector system (BEVS); Authentication: this cell line was characterized using matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry.


90% Grace's + 10% FBS

Cell Culture Collection

Available from Thermo Fisher Scientific (Product#B85502)/or Kerafast (Product#ENH127-FP)


Microorganism Propagation

Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV), Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), Thysanoplusia orichalcea nucleopolyhedrovirus (ThorNPV)

Adventitious Microorganism

Infected with the alphanodavirus TnCLV (Trichoplusi ni cell line virus)


US Patent No. 7179648, Auhtor: Granados, R. R., & Li, G., Date: 20-February-2007; US Patent No. 5300435, Auhtor: Granados, R. R., Date: 25-September-2013

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450