BTI-Tnao38
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
BTI-Tnao38
Accession No.
CVCL_Z252
Stage
Tissue
Initiator(s)
Y. Hashimoto
References
Hashimoto, Y., Zhang, S., & Blissard, G. W. (2010). AO38, A NEW CELL LINE FROM EGGS OF THE BLACK WITCH MOTH, ASCALAPHA ODORATA (LEPIDOPTERA: NOCTUIDAE), IS PERMISSIVE FOR ACMNPV INFECTION AND PRODUCES HIGH LEVELS OF RECOMBINANT PROTEINS. BMC biotechnology, 10(1), 50; Hashimoto, Y., Zhang, S., Zhang, S., Chen, Y. R., & Blissard, G. W. (2012). ERRATUM TO: BTI-TNAO38, A NEW CELL LINE DERIVED FROM TRICHOPLUSIA NI, IS PERMISSIVE FOR ACMNPV INFECTION AND PRODUCES HIGH LEVELS OF RECOMBINANT PROTEINS. BMC biotechnology, 12(1), 12; Wilde, M., Klausberger, M., Palmberger, D., Ernst, W., & Grabherr, R. (2014). TNAO38, HIGH FIVE AND SF9—EVALUATION OF HOST–VIRUS INTERACTIONS IN THREE DIFFERENT INSECT CELL LINES: BACULOVIRUS PRODUCTION AND RECOMBINANT PROTEIN EXPRESSION. Biotechnology letters, 36(4), 743-749; Geisler, C., & Jarvis, D. L. (2018). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein expression and purification, 144, 25-32.
Notes
Origin: this line was originally derived from Ascalapha odorata eggs but was later found to be contaminated with T. ni BTI-Tn-5B1-4 [High Five™] cells (which became the predominant cell type); Unique properties: this cell line expresses secreted alkaline phosphatase (SEAP) and β-galactosidase using an AcMNPV-based baculovirus expression vector.
Medium
TNM-FH + 10% FBS, Sf-900™ III SFM (serum-free medium, wwwthermofishercom)
Cell Culture Collection
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853, USA.
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV) budded virus
