DSIR-HA1179

DSIR-HA1179

Order

Common Name(s)

African black beetle

Primary Designation

DSIR-HA1179

Accession No.

CVCL_Z330

Stage

Tissue

Initiator(s)

A.M. Crawford

References

Crawford, A. M. (1982). A COLEOPTERAN CELL LINE DERIVED FROM HETERONYCHUS ARATOR (COLEOPTERA: SCARABAEIDAE). In Vitro-Plant, 18(10), 813-816; McIntosh, A. H., Grasela, J. J., & Matted, R. L. (1996). IDENTIFICATION OF INSECT CELL LINES BY DNA AMPLIFICATION FINGERPRINTING (DAF). Insect Molecular Biology, 5(3), 187-195; Pushparajan, C., Claus, J. D., Marshall, S. D. G., & Visnovsky, G. (2013). CHARACTERIZATION OF GROWTH AND ORYCTESRHINOCEROS NUDIVIRUS PRODUCTION IN ATTACHED CULTURES OF THE DSIR-HA-1179 COLEOPTERAN INSECT CELL LINE. Cytotechnology, 65(6), 1003-1016.

Notes

Growth characteristics: doubling time = 6 d; Morphology: most cells are spindle-shaped, 25 to 40 µm long and 15 to 20 µm wide; Unique properties: adhere strongly to plastic surfaces and are highly resistant to trypsin and collagenase treatment.

Medium

EX-CELL 420 + 9%FBS

Cell Culture Collection

Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.

Status

Microorganism Propagation

Susceptible to infection by Oryctes rhinoceros nudivirus (OrNV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu