DZNU-Bm-1
Order
Species
Common Name(s)
Silk moth
Primary Designation
DZNU-Bm-1
Accession No.
CVCL_Z082
Stage
Tissue
Initiator(s)
A.M. Khurad
References
Khurad, A. M., Kanginakudru, S., Qureshi, S. O., Rathod, M. K., Rai, M. M., & Nagaraju, J. (2006). A new Bombyx mori larval ovarian cell line highly susceptible to nucleopolyhedrovirus. Journal of Invertebrate Pathology, 92(2), 59-65; Deshmukh C.G., Bahekar R.S. (2013). Comparative in vitro replication and serial passaging of BMNPV in the DZNU-BM-12 and other cell lines. Int. J. Sci. Res. Pub. 3:3.1-3.3; Bahekar R.S. (2018). Growth kinetics and baculovirus productivity of indigenously developed cell line DZNU-Bm-1 of Bombyx mori (L) in different medium. Res. Dir. 6:67-76.
Notes
Morphology: cells are large in number, attached epithelial-like and round refractive cells; after 10 passages the round refractive cells dominated the population; Growth characteristics: Doubling time = 42 h; Karyology: largely diploids and triploids, while a few tetraploids and hexaploids were also observed; Unique properties: the BmNPV was serially passaged in this cell line for five times.
Medium
MGM-448 + 10% FBS +/- 3% heat inactivated B. mori haemolymph; TNM-FH + 10% FBS
Cell Culture Collection
Arun M.Khurad, Department of Zoology, Nagpur University Campus, Nagpur 440 033, India.
csbrnu_ngp@sancharnet.in
Status
Microorganism Propagation
Bombyx mori nucleopolyhedrovirus (BmNPV)
