DZNU-Bm-12
Order
Species
Common Name(s)
Silk moth
Primary Designation
DZNU-Bm-12
Accession No.
CVCL_Z115
Stage
Tissue
Initiator(s)
A.M. Khurad
References
Khurad, A. M., Zhang, M. J., Deshmukh, C. G., Bahekar, R. S., Tiple, A. D., & Zhang, C. X. (2009). A new continuous cell line from larval ovaries of silkworm, Bombyx mori. In Vitro Cellular & Developmental Biology-Animal, 45(8), 414-419; Deshmukh, C. G., & Bahekar, R. S. Comparative in vitro replication and serial passaging of BMNPV in the DZNU-Bm-12 and other cell lines. International Journal of Scientific and Research Publications, 3:3.1-3.3.
Notes
Origin: this cell was developed from ovarian tissue of commercial variety “Kolar Gold” of silkworm, Bombyx mori; Growth characteristics: Doubling time = about 36 and 24 hr; Karyology: chromosome number was near diploid at initial passages and slightly increased at 176th passage, but a few tetraploids and hexaploids; Unique properties: the BmNPV was serially passaged in this cell line for five times.
Medium
MGM-448 + 10% FBS; TNM-FH + 10% FBS
Cell Culture Collection
Arun M.Khurad, Department of Zoology, Nagpur University Campus, Nagpur 440 033, India.
csbrnu_ngp@sancharnet.in
Status
Microorganism Propagation
Bombyx mori nucleopolyhedrovirus (BmNPV)
