Ea.4
Order
Species
Common Name(s)
Salt marsh moth/Saltmarsh caterpillar
Primary Designation
Ea.4
Accession No.
CVCL_Z256
Stage
Tissue
Initiator(s)
O.W. Ogonah/R.R. Granados
References
Ogonah, O. W., Freedman, R. B., Jenkins, N., Patel, K., & Rooney, B. C. (1996). Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Nature Biotechnology, 14(2), 197-202.
Notes
Origin: a subclone of BTI-EAA generated by serial dilution; Unique properties: glycosylation abilities of Ea4 cells were more extensive, producing IFN-γ molecules incorporating oligosaccharides with GlcNAc and Gal residues on the outer arms; more extensive N-glycosylation capabilities of the E1 acrea–derived Ea4, compared to current insect cell lines.
Medium
TC100 + 10% FCS (passage for atleast 10 times before use)
Cell Culture Collection
Barrie C. Rooney, Research School of Biosciences, Biological Laboratory, The University of Kent, Canterbury, UK.
B.C.Rooney@ukc.ac.uk
Status
Microorganism Propagation
Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
