FPMI-CF-70
Order
Species
Common Name(s)
Spruce Budworm
Primary Designation
FPMI-CF-70
Accession No.
CVCL_Z472
Stage
Tissue
Initiator(s)
G.T. Harvey and S.S. Sohi
References
Harvey, G. T., & Sohi, S. S. (1985). Isozyme characterization of 28 cell lines from five insect species. Canadian Journal of Zoology, 63(10), 2270-2276; Harvey, G. T., & Sohi, S. S. (1989). Isozyme characterization of 8 hymenopteran and 20 lepidopteran cell lines. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi J.); pp.71-75; CRC Press; Boca Raton; Sohi, S. S., Palli, S. R., Cook, B. J., & Retnakaran, A. (1995). Forest insect cell lines responsive to 20-hydroxyecdysone and two nonsteroidal ecdysone agonists, RH-5849 and RH-5992. Journal of Insect Physiology, 41(6), 457-464; Tremblay, G. B., Sohi, S. S., Retnakaran, A., & MacKenzie, R. E. (1995). NAD‐dependent methylenetetrahydrofolate dehydrogenase‐methenyltetrahydrofolate cyclohydrolase is targeted to the cytoplasm in insect cell lines. FEBS letters, 368(1), 177-182; Feng, Q., Arif, B. M., Palli, S. R., Sohi, S. S., & Retnakaran, A. (2001). Molecular modifications of baculoviruses for the control of forest insect pests. Advances in Virus Research, 57, 263-290.
Notes
Authentication: isozyme patterns were used for reliable identification; Unique properties: cell lines express high levels of NMDMC (NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase); Many of the clones have been reported by "Great Lakes Forestry Centre", which are as follows: FPMI-CF-70 Clones (Clone B5 Cf DEF will infect 19% of these cells ); Clone B6 Cf DEF will infect 67% of these cells; Clone A4 CfDEF will infect 19% of these cells.
Medium
90% Grace's insect medium + 10%FBS
Cell Culture Collection
D. Doucet, Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada.
daniel.doucet@canada.ca
Status
Microorganism Propagation
Choristoneura fumiferalza multinucleocapsid nucleopolyhedrovirus (cfMNPV); Choristoneura fumiferana defective nucleopolyhedrovirus (CfDEFNPV)
