FPMI-MD-108
Order
Species
Common Name(s)
Forest tent caterpillar
Primary Designation
FPMI-MD-108
Accession No.
CVCL_Z482
Stage
Tissue
Initiator(s)
S. S. Sohi
References
Sohi, S. S. (1973). Establishment of cultures of Malacosoma disstria HÜBNER (Lepidoptera: Lasiocampidae) hemocytes in a hemolymph-free medium. In Proc. 3rd Int. Colloq. Invertebr. Tissue Culture, Smolenice, Czechoslovakia (Eds: Rehacek J., Blaskovic D., Hink W.F.) (pp. 27-39); Krywienczyk, J., & Sohi, S. S. (1976). Serologic characterization and identification of four lepidopteran cell lines. Canadian Journal of Zoology, 54(9), 1559-1564; Volkman, L. E., & Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Appl. Environ. Microbiol., 44(1), 227-233; Lapointe, J. F., Dunphy, G. B., Giannoulis, P., Mandato, C. A., Nardi, J. B., Gharib, O. H., & Niven, D. F. (2011). Cell lines, MD108 and MD66, from hemocytes of Malacosoma disstria (Lepidoptera) display aspects of plasma-free innate non-self activities. Journal of Invertebrate Pathology, 108(3), 180-193.
Notes
Morphology: the majority of these cells are fibroblastic, the remainder are ovo-elliptical; Growth characteristics: cells are freely suspended in the medium; Unique properties: cells display aspects of plasma-free innate non-self activities; Authentication: Identity of this cell line is confirmed using a double diffusion immunoassay.
Medium
Grace’s medium supplemented with heat-inactivated fetal calf serum (8% v/v) and tryptose broth (25 g/l medium)
Cell Culture Collection
Daniel Doucet, Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada.
Dan.Doucet@NRCan.gc.ca
Status
Microorganism Propagation
Autographa californica polyhedrovirus (AcMNPV)
