FRI-AnCo-5B

FRI-AnCo-5B

Order

Species

Common Name(s)

Larger striated chafer

Primary Designation

FRI-AnCo-5B

Accession No.

CVCL_Z269

Stage

Tissue

Initiator(s)

J. Mitsuhashi

References

Mitsuhashi, J. (1989). A continuous cell line from a beetle, Anomala costata Hope (Coleoptera, Scarabaeidae). Journal of the Japanese Forestry Society, 71(12), 491-496.

Notes

Morphology: cells are polygonal in shape, and their diameters are about 15 µm; cells looked like epithelial cells and formed cell sheets; Growth characteristics: population doubling time ~4.5 d; Karyology: diploid; Unique properties: population mostly consisted of cells which had many large vacuoles in their cytoplasm; Authentication: distinguishable from other insect cell lines by the isozyme patterns of isocitrate dehydrogenase (ICD), malic enzyme (ME) , phosphoglucose isomerase (PGI) and phosphoglucomutase (PGM).

Medium

MGM-443 + 10% FBS

Cell Culture Collection

National Institute of Agrobiological Resources, Yatabe, Ibaraki, Japan.

Status

Microorganism Propagation

Susceptible to infection by Sericesthis and Costelytra iridescent viruses

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu