IAL-TND1
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
IAL-TND1
Accession No.
CVCL_Z391
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., Miller, S. G., & Oberlander, H. (1982). Establishment of a cell line from lepidopteran wing imaginal discs: induction of newly synthesized proteins by 20-hydroxyecdysone. Proceedings of the National Academy of Sciences, 79(8), 2589-2593; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology, v.82 no.2 pp. 129; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Lynn, D. E. (2007). Available lepidopteran insect cell lines. In: Methods In Molecular Biology, Vol. 338: Baculovirus and Insect Cell Expression Protocols (Ed: D. W. Murhammer), pp. 117-137, Humana Press Inc, Totowa, NJ.
Notes
Authentication: two-dimensional electrophoresis of methionine-labeled proteins revealed similar patterns for LAL-TND1 cells and whole T. ni imaginal wing discs; Unique properties: exposure to the insect steroid hormone 20-hydroxyecdysone stimulated the synthesis of the same new proteins in both whole wing discs and IAL-TND1 cell vesicles.
Medium
IPL-52B-P, available from US Biological Life Sciences (https://wwwusbionet/media/I8451)
Cell Culture Collection
IIBBL-Invasive Insect Biocontrol & Behavior Laboratory USDA, ARS, Beltsville, Maryland, USA.
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV or AnfaMNPV), but is less susceptible to Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV); susceptible to Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV)
