IOZCAS-Spex-12
Order
Species
Common Name(s)
Beet armyworm
Primary Designation
IOZCAS-Spex-12
Accession No.
CVCL_9U10
Stage
Tissue
Initiator(s)
X. Li
References
Li, X., Qin, Q., Zhang, N., Zhu, W., Zhang, J., Wang, H., Miao, L. and Zhang, H. (2012). A NEW INSECT CELL LINE FROM PUPAL OVARY OF SPODOPTERA EXIGUA ESTABLISHED BY STIMULATION WITH N-METHYL-N′-NITRO-N-NITROSOGUANIDINE (MNNG). In Vitro Cellular & Developmental Biology-Animal, 48(5), 271-275.
Notes
Morphology: this cell line consists of a mixture of three types of cells, including spherical, spindle-shaped, and oval cells; Growth characteristics: population doubling time = 71 h; Authentication: DNA amplification fingerprinting polymerase chain reaction analysis confirmed that the new cell line originated from S. exigua; Unique properties: established by treating primary cells with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG).
Medium
TNM-FH + 10% FBS
Cell Culture Collection
H. Zhang, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, China.
zhanghuan@ioz.ac.cn
Status
Microorganism Propagation
Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Spodoptera litura nucleopolyhedrovirus (SlNPV)
