IOZCAS-Spex-II
Order
Species
Common Name(s)
Beet armyworm
Primary Designation
IOZCAS-Spex-II
Accession No.
CVCL_0A49
Stage
Tissue
Initiator(s)
Z. Zhang
References
Zhang Z. (2006). STUDIES ON PROLIFICATION OF CELLS OF INSECT FAT BODIES IN VITRO AND ESTABLISHMENT OF SEVEN CELL LINES SUSCEPTIBLE TO HOMOLOGOUS VIRUS. Thesis PhD (2006), Chinese Academy of Forestry, China; Zhang, H., Zhang, Y.A., Qin, Q., Li, X., Miao, L., Wang, Y., Yang, Z. and Ding, C. (2006). NEW CELL LINES FROM LARVAL FAT BODIES OF SPODOPTERA EXIGUA: CHARACTERIZATION AND SUSCEPTIBILITY TO BACULOVIRUSES (LEPIDOPTERA: NOCTUIDAE). Journal of Invertebrate Pathology, 91(1), pp.9-12; Zhang, H., Li, X., Miao, L., Wang, H.T., Zhang, A.J., Du, H., Yang, Q. and Qin, Q.L. (2009). ESTABLISHMENT OF CELL LINES FROM THE FAT BODIES OF SPODOPTER EXIGUA LARVAE [J]. Chinese Journal of Cell Biology, 5; Zhang, H., Zhang, Y.A., Qin, Q., Li, X., Miao, L., Wang, Y., Qu, L., Zhang, A. and Yang, Q. (2009). A CELL STRAIN CLONED FROM SPODOPTERA EXIGUA CELL LINE (IOZCAS-SPEX-II) HIGHLY SUSCEPTIBLE TO S. EXIGUA NUCLEOPOLYHEDROVIRUS INFECTION. In Vitro Cellular & Developmental Biology-Animal, 45(5-6), pp.201-204.
Notes
Morhphology: spherical cells is predominant among the various cell types and measures approximately 15 µm in diameter; other cell type is spindle-shaped (12.7%), ranging from 16.0 to 41.9µm (mean 25.3 µm) in length and 8.4 to 16.µm (mean 11.2 µm) in width; Karyology: cell line mainly composed of tetraploid cells with chromosome numbers ranging from 116 to 131 (n = 31); Authentication: cell line identity is confirmed to have originated from the S. exigua by DAF–PCR.
Medium
TNM-FH medium + 10% FBS (fetal bovine serum)
Cell Culture Collection
H. Zhang, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, China.
zhanghuan@ioz.ac.cn
Status
Microorganism Propagation
Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
