IPLB-HvE1a
Order
Species
Common Name(s)
Tobacco budworm
Primary Designation
IPLB-HvE1a
Accession No.
CVCL_Z110
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., & Shapiro, M. (1998). New cell lines from Heliothis virescens: characterization and susceptibility to baculoviruses. Journal of Invertebrate Pathology, 72(3), 276-280; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82 (2), 129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49.
Notes
Morphology: predominantly fibroblast and a few cells are narrow and form a web-like network; Unique properties: HvE1a strains subcultured using trypsinization.
Medium
BML-TC/10 medium
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV), Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV 1), Rachoplusia ou multiple nucleopolyhedrovirus (RoMNPV) and Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV); produces large number of occlusion bodies.
