IPLB-HvE6a-lt
Order
Species
Common Name(s)
Tobacco budworm
Primary Designation
IPLB-HvE6a-lt
Accession No.
CVCL_Z114
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., & Shapiro, M. (1998). New cell lines from Heliothis virescens: characterization and susceptibility to baculoviruses. Journal of Invertebrate Pathology, 72(3), 276-280; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82 (2), 129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49.
Notes
Morphology: these cells are predominantly fibroblast like; cells are broader and are closely aligned to each other in parallel arrays; Growth characteristics: these cell line are maintained at low temperatures (17°C); subcultured using trypsinization; Authentication: similarity of the isozyme patterns of these cell to HvT1, another H. virescens line maintained in our lab, indicates that they are derived from H. virescens.
Medium
BML-TC/10 medium
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV), Autographa californica multiple nucleopolyhedrovirus (AcMNPV) , Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV 1), Rachoplusia ou multiple nucleopolyhedrovirus (RoMNPV) and Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV); produces large number of occlusion bodies; no cytopathology (CPE) observed for Orgyia leucostigma multiple nucleopolyhedrovirus (OlMNPV)
