IPLB-HvE6s
Order
Species
Common Name(s)
Tobacco budworm
Primary Designation
IPLB-HvE6s
Accession No.
CVCL_Z574
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., & Shapiro, M. (1998). New cell lines from Heliothis virescens: characterization and susceptibility to baculoviruses. Journal of Invertebrate Pathology, 72(3), 276-280; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82 (2), 129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49.
Notes
Morphology: During early passages, the HvE6 line showed heterogeneous morphologies between the attached and suspended cell types and separate strains were isolated from this by selecting for nonattachment, respectively, and were designated as IPLB-HvE6s; Growth characteristics: these cell line are maintained at low temperatures (17°C).
Medium
BML-TC/10 medium
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV), Autographa californica multiple nucleopolyhedrovirus (AcMNPV) , Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV 1), Rachoplusia ou multiple nucleopolyhedrovirus (RoMNPV) and Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV); produces large number of occlusion bodies; no cytopathology (CPE) observed for Orgyia leucostigma multiple nucleopolyhedrovirus (OlMNPV)
