IPLB-HZ-1075
Order
Species
Common Name(s)
cotton bollworm
Primary Designation
IPLB-HZ-1075
Accession No.
CVCL_Z209
Stage
Tissue
Initiator(s)
R. H. Goodwin
References
Goodwin, R. H., Topkins, G. J., Gettig, R. R., & Adams, J. R. (1982). CHARACTERIZATION AND CULTURE OF VIRUS REPLICATING CONTINUOUS INSECT CELL LINES FROM THE BOLLWORM, HELIOTHIS ZEA (BODDIE). In Vitro-Plant, 18(10), 843-850; Kurtti, T. J., TSANG, K. R., & BROOKS, M. A. (1983). THE SPREAD OF INFECTION BY THE MICROSPORIDAN, NOSEMA DISSTRIAE, IN INSECT CELL LINES 1. The Journal of Protozoology, 30(4), 652-657; INTRINSIC RESISTANCE TO THE LETHAL EFFECTS OF X-IRRADIATION IN INSECT AND ARACHNID CELLS; CHARACTERIZATION OF CLONAL POPULATIONS OF THE HELIOTHIS ZEA CELL LINE IPLB-HZ 1075; Corsaro, B. G., & Fraser, M. J. (1987). CHARACTERIZATION OF CLONAL POPULATIONS OF THE HELIOTHIS ZEA CELL LINE IPLB-HZ 1075. In vitro cellular & developmental biology, 23(12), 855-862; Corsaro, B. G., DiRenzo, J., & Fraser, M. J. (1989). TRANSFECTION OF CLONED HELIOTHIS ZEA CELL LINES WITH THE DNA GENOME OF THE HELIOTHIS ZEA NUCLEAR POLYHEDROSIS VIRUS. Journal of virological methods, 25(3), 283-291.
Notes
Morphology: cells are fibroblastic-like; Authentication: serological (micro-immunodiffusion) and isozyme (phosphoglucoisomerase and peptidase:glycyl-leucine) techniques were used to confirm species-of-origin identity; Microbial presence: mycoplasma-free.
Medium
IPL-41 26g/l Tryptose PO4
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Helicoverpa zea single nucleopolyhedrovirus (HzSNPV), produces occlusion bodies; able to replicate Nosema disstriae
