IPLB-Ld-652Y
Order
Species
Common Name(s)
Gypsy moth
Primary Designation
IPLB-Ld-652Y
Accession No.
CVCL_Z381
Stage
Tissue
Initiator(s)
R. H. Goodwin
References
Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1978). Gypsy moth cell lines divergent in viral susceptibility. In vitro, 14(6), 485-494; Gundersen-Rindal, D., Slack, J. M., & Lynn, D. E. (2000). Transfection of Lymantria dispar insect cell lines. Methods in cell science, 22(4), 257-263; Wu, C. Y., Lin, H. F., Wang, C. H., & Lo, C. F. (2011). Identification of insect cell lines and cell‐line cross‐contaminations by nuclear ribosomal ITS sequences. Journal of Applied Entomology, 135(8), 601-610; Carrillo-Tripp, J., Krueger, E. N., Harrison, R. L., Toth, A. L., Miller, W. A., & Bonning, B. C. (2014). Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans. Journal of General Virology, 95(10), 2285-2296.
Notes
Unique properties: this cell line could be used as a superior choice for expression studies (cationic lipid-mediated transfection) or systems requiring L. dispar-derived cells; Distinguish characteristics: microimmunodiffusion assay and PCR-RFLP is performed to differentiate this cell line from other lepidopteran insects and pupal extracts from L. dispar; subline: IPLB-Ld-652Y/Gf.
Medium
IPL-41 26% Tryptose PO4 + 10% FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
Adventitious Microorganism
Lymantria dispar iflavirus 1 (LdIV1)
