IPLB-Ld-67
Order
Species
Common Name(s)
Gypsy moth
Primary Designation
IPLB-Ld-67
Accession No.
CVCL_Z385
Stage
Tissue
Initiator(s)
R. H. Goodwin
References
Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1978). Gypsy moth cell lines divergent in viral susceptibility. In vitro, 14(6), 485-494; Hink, W. F. (1980). The 1979 compilation of invertebrate cell lines and culture media. In: Invertebrate Systems In Vitro (Eds: Kurstak, E., Maramorosch, K., Duebendorfer, A.), pp.553-578, Elsevier, Oxford.
Notes
Morphology: this cell line is mostly spherical to fibroblastic ranging from about 20 to 30 µm in diameter; Growth characteristics: cells grow in a loose monolayer with considerable clumping in dense cultures; Authentication: the microimmunodiffusion assay is performed to differentiate this cell line from other lepidopteran insects and pupal extracts from L. dispar; Subline: IPLB-Ld-67AB and IPLB-Ld-67AC.
Medium
IPL-41 + 26/gl Tryptose PO4 + 10% FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
