IPLB-LdEIta
Order
Species
Common Name(s)
Gypsy moth
Primary Designation
IPLB-LdEIta
Accession No.
CVCL_Z099
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., Dougherty, E. M., McClintock, J. T., & Loeb, M. (1988). Development of cell lines from various tissues of Lepidoptera. In: Invertebrate and Fish Tissue Culture (eds. Kuroda, Y., Kurstak, E., Maramorosch, K.); pp.239-242; Japan Sci. Soc.; Tokyo; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Lynn, D. E. (1999). Comparison of cell line maintenance procedures on insect cells used for producing baculoviruses. In Vitro Cellular & Developmental Biology-Animal, 35(5), 248-251; Gundersen-Rindal, D., Slack, J. M., & Lynn, D. E. (2000). Transfection of Lymantria dispar insect cell lines. Methods in Cell Science, 22(4), 257-263; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82 (2), 129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49.
Notes
Unique properties: this cell line adapted quickly to new medium and grew better in the serum-free formula than in serum-containing medium.
Medium
TC100 + Stock P, 1:10 FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV).
