IPLB-LdEp
Order
Species
Common Name(s)
Gypsy moth
Primary Designation
IPLB-LdEp
Accession No.
CVCL_Z102
Stage
Tissue
Initiator(s)
D. E. Lynn
References
Lynn, D. E., Dougherty, E. M., McClintock, J. T., & Loeb, M. (1988). Development of cell lines from various tissues of Lepidoptera. In: Invertebrate and fish tissue culture (eds. Kuroda, Y., Kurstak, E., Maramorosch, K.); pp.239-242; Japan Sci. Soc.; Tokyo.; Gundersen-Rindal, D., Slack, J. M., & Lynn, D. E. (2000). Transfection of Lymantria dispar insect cell lines. Methods in Cell Science, 22(4), 257-263; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82 (2), 129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Carrillo-Tripp, J., Krueger, E. N., Harrison, R. L., Toth, A. L., Miller, W. A., & Bonning, B. C. (2014). Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans. Journal of General Virology, 95(10), 2285-2296.
Notes
Unique properties: this cell line could be used as a superior choice for expression studies (cationic lipid-mediated transfection) or systems requiring L. dispar-derived cells; Sublines: IPLB-LdEp/GfA, IPLB-LdEp/Gi, IPLB-LdEp/GfB, LdEp/GpA+Bc, IPLB-LdEp/GpV and IPLB-LdEp/GpVV.
Medium
IPL-52B + IPL-76 peptones 9% FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV) (AcMNPV), Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV), and Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)
