IPLB-Sf-1254
Order
Species
Common Name(s)
Fall armyworm
Primary Designation
IPLB-Sf-1254
Accession No.
CVCL_Z439
Stage
Tissue
Initiator(s)
J. Vaughn
References
Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. J. I. V. (1977). THE ESTABLISHMENT OF TWO CELL LINES FROM THE INSECT SPODOPTERA FRUGIPERDA (LEPIDOPTERA; NOCTUIDAE). In vitro, 13(4), 213-217; Koval T.M. (1983). INTRINSIC RESISTANCE TO THE LETHAL EFFECTS OF X-IRRADIATION IN INSECT AND ARACHNID CELLS. Proc. Natl. Acad. Sci. U.S.A. 80:4752-4755; Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J. (1991). EXPRESSION OF THREE RECOMBINANT PROTEINS USING BACULOVIRUS VECTORS IN 23 INSECT CELL LINES. Biotechnol. Prog. 7:9-14.
Notes
Morphology: this cell line contained the same cell types as Sf21: small spherical cells averaging 19.5 µm in diameter; fibroblast-like cells averaging 116 µm by 20 µm; and polymorphic cells averaging 48 µm by 30 µm; Growth characteristics: population doubling time of 36 hr in IPL-40/45 or 24 hr in TNM-FH.
Medium
IPL-41 (+ 026% Tryptose Phosphate Broth) or TNM-FH + 10% FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Susceptible to infection by Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Spodoptera frugiperda multiple nucleopolyhedrovirus (AgMNPV), and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV)
