IPLB-Sf21

IPLB-Sf21

Order

Common Name(s)

Fall armyworm

Primary Designation

IPLB-Sf21

Accession No.

CVCL_0518

Stage

Tissue

Initiator(s)

J. Vaughn

References

Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. J. I. V. (1977). The establishment of two cell lines from Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro, 13(4), 213-217; Volkman, L. E., & Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Applied and Environmental Microbiology, 44(1), 227-233; Mitsuhashi, J. (1989). NUTRITIONAL REQUIREMENTS OF INSECT CELLS IN VITRO. Invertebrate cell system applications, 1; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). EXPRESSION OF THREE RECOMBINANT PROTEINS USING BACULOVIRUS VECTORS IN 23 INSECT CELL LINES. Biotechnology progress, 7(1), 9-14; Wickham, T. J., Davis, T., Granados, R. R., Shuler, M. L., & Wood, H. A. (1992). SCREENING OF INSECT CELL LINES FOR THE PRODUCTION OF RECOMBINANT PROTEINS AND INFECTIOUS VIRUS IN THE BACULOVIRUS EXPRESSION SYSTEM. Biotechnology Progress, 8(5), 391-396; Davis, T. R., Wickham, T. J., McKenna, K. A., Granados, R. R., Shuler, M. L., & Wood, H. A. (1993). COMPARATIVE RECOMBINANT PROTEIN PRODUCTION OF EIGHT INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 29(5), 388-390; Lulin, L., & Quhou, C. (1995). STORAGE OF INSECT CELL LINES BY A SIMPLE METHOD. Hua Zhong shi fan da xue xue bao. Zi ran ke xue ban= Journal of Central China Normal University. Natural Sciences Edition, 29(4), 511-512; McIntosh, A. H., Grasela, J. J., & Matted, R. L. (1996). IDENTIFICATION OF INSECT CELL LINES BY DNA AMPLIFICATION FINGERPRINTING (DAF). Insect Molecular Biology, 5(3), 187-195; Keith, M. B., Farrell, P. J., Iatrou, K., & Behie, L. A. (1999). SCREENING OF TRANSFORMED INSECT CELL LINES FOR RECOMBINANT PROTEIN PRODUCTION. Biotechnology progress, 15(6), 1046-1052; Grasela, J. J., Mcintosh, A. H., Goodman, C. L., Wilson, L. E., & King, L. A. (2000). EXPRESSION OF THE GREEN FLUORESCENT PROTEIN CARRIED BY AUTOGRAPHA CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS IN INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 36(3), 205-210; Kariuki, C. W., McINTOSH, A. H., & Goodman, C. L. (2000). IN VITRO HOST RANGE STUDIES WITH A NEW BACULOVIRUS ISOLATE FROM THE DIAMONDBACK MOTH PLUTELLA XYLOSTELLA (L.)(PLUTELLIDAE: LEPIDOPTERA). In Vitro Cellular & Developmental Biology-Animal, 36(4), 271-276; Lynn, D. E. (2003). COMPARATIVE SUSCEPTIBILITIES OF TWELVE INSECT CELL LINES TO INFECTION BY THREE BACULOVIRUSES; Grasela, J. J., & Mcintosh, A. H. (2003). APPLICATION OF INTER-SIMPLE SEQUENCE REPEATS TO INSECT CELL LINES: IDENTIFICATION AT THE CLONAL ANDTISSUE-SPECIFIC LEVEL. In Vitro Cellular & Developmental Biology-Animal, 39(8-9), 353-363; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). BACULOVIRUS-BASED EXPRESSION OF AN INSECT VIRAL PROTEIN IN 12 DIFFERENT INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Mcintosh, A. H., Grasela, J. J., & Popham, H. J. (2005). ACMNPV IN PERMISSIVE, SEMIPERMISSIVE, AND NONPERMISSIVE CELL LINES FROM ARTHROPODA. In Vitro Cellular & Developmental Biology-Animal, 41(8-9), 298-304; Ma, H., Galvin, T. A., Glasner, D. R., Shaheduzzaman, S., & Khan, A. S. (2014). IDENTIFICATION OF A NOVEL RHABDOVIRUS IN SPODOPTERA FRUGIPERDA CELL LINES. Journal of Virology, 88(12), 6576-6585; Geisler, C., & Jarvis, D. L. (2018). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein expression and purification, 144, 25-32.

Notes

Morphology: this cell line contained three recognizable cell types: small spherical cells averaging 19.5 µm in diameter; fibroblast-like cells averaging 116 µm by 20 µm; and polymorphic cells averaging 48 µm by 30 µm; Growth characteristics: population doubling time of 26 to 30 hr in IPL40/45 medium.

Medium

EX-CELL 420 or TC-100 + 10% FBS or a variety of serum-free media from different vendors

Cell Culture Collection

Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA; Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.

Status

Microorganism Propagation

Susceptible to infection by Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Plutella xylostella multiple nucleopolyhedrovirus (PxMNPV), Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), Spodoptera littoralis multiple nucleopolyhedrovirus (SpliNPV), and Thysanoplusia orichalcea multiple nucleopolyhedrovirus (ThorMNPV)

Adventitious Microorganism

Rhabdovirus

Patents

Please check again for patents

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu