Common Name(s)

Cabbage looper

Primary Designation


Accession No.





R. Rochford


Rochford, R., Dougherty, E. M., & Lynn, D. E. (1984). Establishment of a cell line from embryos of the cabbage looper, Trichoplusia ni (HUBNER). In Vitro, 20(11), 823-825; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Lynn, D. E. (1992). Improved efficiency in determining the titer of the Autographa californica baculovirus nonoccluded virus. Biotechniques, 13(2), 282-285; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82:129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Lynn, D (2007). Available lepidopteran insect cell lines. In: Methods In Molecular Biology, Vol. 338: Baculovirus and Insect Cell Expression Protocols (Ed: D. W. Murhammer), pp. 117-137, Humana Press Inc, Totowa, NJ.


Morphology: this cell line consists of a mixture of cell types, including neuroblast-like, myoblast-like, and epithelial-like that exist either as vesicles or in monolayers and either attached or in suspension; Karyology: chromosomes are condensed short rods and spheres, which are characteristic of lepidopteran cell lines; chromosome numbers ranged between 40 and 76 (2N for T. ni = 62); Authentication: isozyme pattern analysis is used to differentiate the cell line from other lepidopteran cell lines.


TNM-FH medium + 20% FBS

Cell Culture Collection

Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.


Microorganism Propagation

Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV or AnfaMNPV), Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450