IPLB-TN-R2
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
IPLB-TN-R2
Accession No.
CVCL_Z096
Stage
Tissue
Initiator(s)
R. Rochford
References
Rochford, R., Dougherty, E. M., & Lynn, D. E. (1984). Establishment of a cell line from embryos of the cabbage looper, Trichoplusia ni (HUBNER). In Vitro, 20(11), 823-825; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Lynn, D. E. (1992). Improved efficiency in determining the titer of the Autographa californica baculovirus nonoccluded virus. Biotechniques, 13(2), 282-285; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses. Journal of Invertebrate Pathology 82:129-131; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Lynn, D (2007). Available lepidopteran insect cell lines. In: Methods In Molecular Biology, Vol. 338: Baculovirus and Insect Cell Expression Protocols (Ed: D. W. Murhammer), pp. 117-137, Humana Press Inc, Totowa, NJ.
Notes
Morphology: this cell line consists of a mixture of cell types, including neuroblast-like, myoblast-like, and epithelial-like that exist either as vesicles or in monolayers and either attached or in suspension; Karyology: chromosomes are condensed short rods and spheres, which are characteristic of lepidopteran cell lines; chromosome numbers ranged between 40 and 76 (2N for T. ni = 62); Authentication: isozyme pattern analysis is used to differentiate the cell line from other lepidopteran cell lines.
Medium
TNM-FH medium + 20% FBS
Cell Culture Collection
Robert Harrison, USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, USA.
robert.l.harrison@usda.gov
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV or AnfaMNPV), Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV)
