IPRI-CF-1
Order
Species
Common Name(s)
Spruce Budworm
Primary Designation
IPRI-CF-1
Accession No.
CVCL_Z473
Stage
Tissue
Initiator(s)
D.E. Johnson
References
Johnson, D. E. (1984). Selection for resistance to Bacillus thuringiensis δ-endotoxin in an insect cell line (Choristoneura fumiferana). Experientia, 40(3), 274-275; Harvey, G. T., & Sohi, S. S. (1985). Isozyme characterization of 28 cell lines from five insect species. Canadian Journal of Zoology, 63(10), 2270-2276; Harvey, G. T., & Sohi, S. S. (1989). Isozyme characterization of 8 hymenopteran and 20 lepidopteran cell lines. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi J.); pp.71-75; CRC Press; Boca Raton; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Sohi, S. S., Palli, S. R., Cook, B. J., & Retnakaran, A. (1995). Forest insect cell lines responsive to 20-hydroxyecdysone and two nonsteroidal ecdysone agonists, RH-5849 and RH-5992. Journal of Insect Physiology, 41(6), 457-464; Tremblay, G. B., Sohi, S. S., Retnakaran, A., & MacKenzie, R. E. (1995). NAD‐dependent methylenetetrahydrofolate dehydrogenase‐methenyltetrahydrofolate cyclohydrolase is targeted to the cytoplasm in insect cell lines. FEBS letters, 368(1), 177-182; Feng, Q., Arif, B. M., Palli, S. R., Sohi, S. S., & Retnakaran, A. (2001). Molecular modifications of baculoviruses for the control of forest insect pests. Advances in Virus Research, 57, 263-290.
Notes
Unique properties: resistant to the entomocidal protein ( δ-endotoxin) extracted from Bacillus thuringiensis crystals; CF-1 lines showed little or no morphological response to the nonsteroidal ecdysone agonist; Authentication: isozyme profiles of cell line from the same species showed unique characteristics that will be useful in monitoring their identity.
Medium
90% Grace's insect medium + 10% FBS
Cell Culture Collection
D. Doucet, Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada.
daniel.doucet@canada.ca
