IPRI-CF-16T
Order
Species
Common Name(s)
Spruce Budworm
Primary Designation
IPRI-CF-16T
Accession No.
CVCL_WV94
Stage
Tissue
Initiator(s)
G.B. Tremblay
References
Tremblay, G. B., Sohi, S. S., Retnakaran, A., & MacKenzie, R. E. (1995). NAD‐dependent methylenetetrahydrofolate dehydrogenase‐methenyltetrahydrofolate cyclohydrolase is targeted to the cytoplasm in insect cell lines. FEBS letters, 368(1), 177-182; Feng, Q., Arif, B. M., Palli, S. R., Sohi, S. S., & Retnakaran, A. (2001). Molecular modifications of baculoviruses for the control of forest insect pests. Advances in virus research, 57, 263-290.
Notes
Unique properties: cell lines express high levels of NMDMC (NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase); this cell line has increased attachment adherence compared to the parent cell line.
Medium
90% Grace's insect medium + 10% FBS
Cell Culture Collection
D. Doucet, Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada.
daniel.doucet@canada.ca
Status
Microorganism Propagation
Choristoneura fumiferalza multinucleocapsid nucleopolyhedrovirus (cfMNPV)
