IZD-MB-0503
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
IZD-MB-0503
Accession No.
CVCL_C411
Stage
Tissue
Initiator(s)
H. G. Miltenburger
References
Miltenburger, H. G., David, P., Mahr, U., & Zipp, W. (1976). Establishment of lepidopteran cell lines and in vitro replication of insect-pathogenic virues: 1. Mamestra brassicae cell lines and NPV-replication. J. Appl. Entomol. 82:306-323; Hink, W. F. (1980). The 1979 compliation of invertebrate cell lines and culture media. In: Invertebrate Systems in Vitro (Editors: Maramorosch, K.; Duebendorfer, A.), pp.553-578, Elsevier; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Davis, T. R., Wickham, T. J., McKenna, K. A., Granados, R. R., Shuler, M. L., & Wood, H. A. (1993). Comparative recombinant protein production of eight insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 29(5), 388-390; McIntosh, A. H., Grasela, J. J., & Matted, R. L. (1996). Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Molecular Biology, 5(3), 187-195; Mandrioli, M., & Volpi, N. (2003). The genome of the lepidopteran Mamestra brassicae has a vertebrate-like content of methyl-cytosine. Genetica, 119(2), 187-191; Lynn, D. (2007). Available lepidopteran insect cell lines. In: Methods in Molecular Biology, Vol 338: Baculovirus and Insect Cell Expression Protocols (Ed. Murhammar, D. W.), Humana Press Inc., Totowa, NJ, pp 117-137
Notes
Growth characteristics: doubling time was 12-14 hr for attached cultures, this line could be cultured continuously in suspension by periodic harvesting and addition of fresh medium with maximum cell densities of 3 x 10e6 cells/mL and a population doubling time of 17 h; Unique properties: a baculovirus expression was used to produce 3 recombinant proteins and this cell line produced the highest yield of pseudorabies virus glycoprotein (gp50T), human plasminogen (HPg), and β-galactosidase β-gal).
Medium
Grace's insect medium + 5% FCS
Status
Microorganism Propagation
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Mamestra brassicae multiple nucleopolyhedrovirus nucleopolyhedrovirus (MbMNPV)
