IZD-MB-2506

IZD-MB-2506

Order

Common Name(s)

Cabbage moth

Primary Designation

IZD-MB-2506

Accession No.

CVCL_AR83

Stage

Tissue

Initiator(s)

H. G. Miltenburger

References

Miltenburger, H. G., David, P., Mahr, U., & Zipp, W. (1976). Establishment of lepidopteran cell lines and in vitro replication of insect-pathogenic virues: 1. Mamestra brassicae cell lines and NPV-replication. Journal of Applied Entomology 82:306-323; Lynn, D. (2007). Available lepidopteran insect cell lines. In: Methods in Molecular Biology, Vol 338: Baculovirus and Insect Cell Expression Protocols (Ed. Murhammar, D. W.), Humana Press Inc., Totowa, NJ, pp 117-141

Notes

Growth characteristics: doubling time was ~40 hr for attached cultures; Karyology: a large numbers of chromosomes were seen per cell.

Medium

Grace's insect medium + 5% FCS

Status

Microorganism Propagation

Autographa californica nucleopolyhedrovirus (AcMNPV) and Mamestra brassicae multiple nucleopolyhedrovirus (MbMNPV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu