IZD-MB-2506
Cabbage moth
IZD-MB-2506
CVCL_AR83
H. G. Miltenburger
Miltenburger, H. G., David, P., Mahr, U., & Zipp, W. (1976). Establishment of lepidopteran cell lines and in vitro replication of insect-pathogenic virues: 1. Mamestra brassicae cell lines and NPV-replication. Journal of Applied Entomology 82:306-323; Lynn, D. (2007). Available lepidopteran insect cell lines. In: Methods in Molecular Biology, Vol 338: Baculovirus and Insect Cell Expression Protocols (Ed. Murhammar, D. W.), Humana Press Inc., Totowa, NJ, pp 117-141
Growth characteristics: doubling time was ~40 hr for attached cultures; Karyology: a large numbers of chromosomes were seen per cell.
Grace's insect medium + 5% FCS
Autographa californica nucleopolyhedrovirus (AcMNPV) and Mamestra brassicae multiple nucleopolyhedrovirus (MbMNPV)
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