MB-020
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
MB-020
Accession No.
CVCL_Z317
Stage
Tissue
Initiator(s)
W. Lehmann
References
Lehmann, W., & Weilepp, M. (1989). Establishment of a cell line from hemocytes of Mamestra brassicae L. and sensitivity to a homologous nuclear polyhedrosis virus. Archiv fuer Phytopathologie und Pflanzenschutz 25:387-401
Notes
(From DSMZ website) Growth characteristics: cells are difficult to culture; split confluent cultures 1:2 every day (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4 °C for up to one week without loss of viability.
Medium
Grace's insect medium + 15% FCS or SF-900 Insect-X Press (protein-free medium)
Status
Microorganism Propagation
Mamestra brassicae multiple nucleopolyhedrovirus (MbMNPV)
