MB-04
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
MB-04
Accession No.
CVCL_Z320
Stage
Tissue
Initiator(s)
W. Lehmann
References
Kurtti, T. J., Tsang, K. R., & Brooks, M. A. (1983). The spread of infection by the microsporidian, Nosema dissitriae, in insect cell lines. The Journal of Protozoology, 30(4), 652-657; Lehmann, W., & Weilepp, M. (1989). Establishment of a cell line from hemocytes of Mamestra brassicae L. and its sensitivity to a homologous nuclear polyhedrosis virus. Archiv fuer Phytopathologie und Pflanzenschutz 25:387-402
Notes
(From DSMZ website) Growth characteristics: cells are difficult to culture! split cultures 1:2 every 2-3 days (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4 °C for up to one week without loss of viability; after thawing cells might have a lag phase of up to 4 weeks
Medium
Grace's insect medium + 15% FCS or + 10% FBS
