MRRL-CH1
Order
Species
Common Name(s)
Tobacco hornworm
Primary Designation
MRRL-CH1
Accession No.
CVCL_Z389
Stage
Tissue
Initiator(s)
P. E. Eide
References
Eide, P. E., Caldwell, J. M., & Marks, E. P. (1975). Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L.). In Vitro, 11(6), 395-399; Hink, W. F. (1980). The 1979 compilation of invertebrate cell lines and culture media. In: Invertebrate Systems in Vitro (Eds. Kurstak, E.; Maramorosch, K.; Duebendorfer, A.), Elsevier; Oxford, pp. 553-578; Volkman, L. E., & Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Appl. Environ. Microbiol., 44(1), 227-233; Koval, T. M. (1983). Intrinsic resistance to the lethal effects of X-irradiation in insect and arachnid cells. Proceedings of the National Academy of Sciences, 80(15), 4752-4755; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14.
Notes
Morphology: this line consists almost entirely of cells of a single type that have the appearance of fibroblasts; when subcultured, the subculture consisted mostly of single cells that attached, divided, and formed loci of spheroidal cells; Growth characterisitcs: doubling time = 8 days; Karyology: lines are probably mixoploid, the diploid number of the hornworm may be 36, and the majority of the cells in the lines are polyploid; Authentication: serologically identical with hornworm tissue.
Medium
TNM-FH + 10% FBS
Status
Microorganism Propagation
Replicates Autographa californica multiple nucleohedrovirus (AcMNPV)
