NIAS-MaBr-85
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
NIAS-MaBr-85
Accession No.
CVCL_Z126
Stage
Tissue
Initiator(s)
J. Mitsuhashi
References
Mitsuhashi, J. (1981). Establishment and some characteristics of a continuous cell line derived from fat bodies of the cabbage armyworm (Lepidoptera, Noctuidae). Development, Growth & Differentiation, 23(1), 63-72; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. In: Invertebrate Cell System Applications, ,Vol. I (Ed: Mitsuhashi J.), pp.3-20, CRC Press, Boca Raton, FL; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Notes
Morphology: cell population is morphologically heterogeneous; spherical cells of about 17 µm diameter are predominant, but there are also spindle-shaped cells and tadpole-shaped cells; Karyology: most of the cells are hexaploid with 180 microchromosomes; Growth characterisitics: doubling time of 60 hours; cells grow in suspension in the medium; Nutritional requirements: cell line consumed beta-alanine.
Medium
Grace's insect medium (supplemented) + 10% FBS
Cell Culture Collection
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Owashi 1-2, Tsukuba, Ibaraki, 305-8634, Japan.
Status
Microorganism Propagation
Chilo iridescent virus (CIV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
