NIAS-MaBr-92
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
NIAS-MaBr-92
Accession No.
CVCL_Z124
Stage
Tissue
Initiator(s)
J. Mitsuhashi
References
Mitsuhashi, J. (1981). Establishment and some characteristics of a continuous cell line derived from fat bodies of the cabbage armyworm (Lepidoptera, Noctuidae). Development, Growth & Differentiation, 23(1), 63-72; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. In: Invertebrate Cell System Applications, ,Vol. I (Ed: Mitsuhashi J.), pp.3-20, CRC Press, Boca Raton, FL; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Notes
Morphology: cells are mostly spherical with diameter of about 20 µm; elongated cells including spindle-shaped cells is the second abundant; Karyology: chromosome numbers varied with the mode of 100 to 120; Growth characterisitics: doubling time = 36 hr; Unique properties: cells could grow in media free of serum and lacking sterols, fatty acids and protein; cell line consumed ammonia and produced glutamine when cultured in MM medium; Nutritional requirements: hemocyte cell lines consumed aspartic acid, asparagine and glutamic acid to a greater extent; Authentication: isoenzyme patterns similar to those of other cell lines from M. brassicae.
Medium
Maramorosch's medium (MM) containing 3% FBS, MM-SF or MTCM-1103
Status
Microorganism Propagation
Chilo iridescent virus (CIV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
