NIAS-MaBr-93
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
NIAS-MaBr-93
Accession No.
CVCL_Z125
Stage
Tissue
Initiator(s)
J. Mitsuhashi
References
Mitsuhashi, J., & Shozawa, A. (1985). Continuous cell lines from larval hemocytes of the cabbage armyworm, Mamestra brassicae. Development, Growth & Differentiation, 27(5), 599-606; Koike, M., & Sato, K. (1988). Culture of insect cell lines originated from Mamestra brassicae with autoclaved serum-free medium. In: Invertebrate and Fish Tissue Culture (Editors: Kuroda Y., Kurstak E., Maramorosch K.), pp.7-8; Japan Sci. Soc., Tokyo; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi J.), pp.3-20, CRC Press, Boca Raton, FL; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Notes
Morphology: cells are mostly spherical with diameter of about 20 µm; elongated cells including spindle-shaped cells is the second abundant; Karyology: chromosome numbers of the cell lines varied with the mode of 100 to 120; Growth characterisitics: doubling time = 36 hr; Unique properties: cells could grow in media free of serum and lacking sterols, fatty acids and protein; cell line consumed ammonia and produced glutamine when cultured in MM medium; Nutritional requirements: consumed aspartic acid, asparagine and glutamic acid to a greater extent; Authentication: patterns of isozyme activities similar to those of other cell lines from M. brassicae.
Medium
Maramorosch's medium (MM) containing 3% FBS, MM-SF or MTCM-1104
Status
Microorganism Propagation
Chilo iridescent virus (CIV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
