NIAS-Mb-32
Order
Species
Common Name(s)
Cabbage moth
Primary Designation
NIAS-Mb-32
Accession No.
CVCL_Z123
Stage
Tissue
Initiator(s)
J. Mitsuhashi
References
Mitsuhashi, J. (1977). Establishment and characterization of continuous cell lines from pupal ovaries of the cabbage armyworm, Mamestra brassicae (Lepidoptera, Noctuidae). Development, Growth & Differentiation, 19(4), 337-344; Koike, M., & Sato, K. (1988). Culture of insect cell lines originated from Mamestra brassicae with autoclaved serum-free medium. In: Invertebrate and Fish Tissue Culture (Editors: Kuroda Y., Kurstak E., Maramorosch K.), pp.7-8; Japan Sci. Soc., Tokyo; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi J.), pp.3-20, CRC Press, Boca Raton, FL; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Notes
Morphology: consisted of a heterogeneous cell population, including attached flat cells and spherical free cells; most of the cells had finely branched cytoplasmic processes; Karyology: diploid cells were predominant; Growth characterisitics: doubling time = 48 hr; Unique properties: cells detached comparatively easily from surface by flushing with medium; Nutritional requirements: patterns of amino acid utilization of this cell line was characterized by marked consumption of aspartic acid, cystine, glutamine, histidine, isoleucine, leucine, methionine, tryptophan, tyrosine and valine, and by production of a-alanine and proline.
Medium
Grace’s insect medium + 10% FBS
Status
Microorganism Propagation
Chilo iridescent virus (CIV)
