NIAS-Px-58
Order
Species
Common Name(s)
Asian swallowtail butterfly
Primary Designation
NIAS-Px-58
Accession No.
CVCL_Z132
Stage
Tissue
Initiator(s)
J. Mitsuhashi
References
Mitsuhashi, J. (1973). Establishment of cell lines from the pupal ovaries of the swallow tail, Papilio xuthis Linne: Lepidoptera: Papilionidae. Applied Entomology and Zoology, 8(2), 64-72; Mitsuhashi, J. (1976). Establishment and characterization of cell lines from the pupal ovaries of Papilio xuthus. In: Invertebrate Tissue Culture: Research Applications (Ed.: Maramorosch, K.), Academic Press; New York, pp.13-38; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515; Morikawa, H., Mitsuhashi, J., Ya., Y. (1988). Inter-kingdom electrofusion between tobacco mesopyhyll protoplasts and cultured butterfly cells . Plant Tissue Culture Letters (2):90-92; Mitsuhashi, J., (1982). Continuous cultures of insect cell lines in media free of sera. Appl Ent Zool 17:575-581; Mitsuhashi, J. (1976). Utilization and requirements of amino acids by a cell line derived from the butterfly Papilio xuthus. Journal of Insect Physiology 2:397-40.
Notes
Morphology: cells in this cell line (strain 1, S1) are mostly spherical (20 µm in diameter), fusi-form cells are secondarily predominant (40 µm in diameter); Growth characteristics: doubling time = 60 hr; Unique properties: another cell line strain (NIAS-Px-58 S3) was established by adapting mixed cell population to the culture medium of different composition (MGM-901 + 10% FBS); these cells are mostly tadpole-shaped cells with a long slender tails.
Medium
MGM-431 + 10% FBS or MM (or Schneider's) + 3% FBS
