NIH-SaPe-4
Flesh fly
NIH-SaPe-4
CVCL_Z550
M. Takahashi
Takahashi, M., Mitsuhashi, J., & Ohtaki, T. (1980). Establishment of a cell line from embryonic tissues of the fleshfly, Sarcophaga peregrina (Insecta: Diptera). Development, Growth & Differentiation, 22(1), 11-19; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. Invertebrate cell system applications, CRC Press; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Morphology: cells were heterogeneous in morphology; Karyotype: diploid and their chromosomes consisted of 4 metacentric, 6 sub‐metacentric and 2 micro chromosomes; Growth characteristics: population doubling time is 30 hr.
Grace's Insect medium + 10%FBS
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