NIV-HA-197
Order
Species
Common Name(s)
cotton bollworm
Primary Designation
NIV-HA-197
Accession No.
CVCL_Z307
Stage
Tissue
Initiator(s)
A. B. Sudeep
References
Sudeep, A. B., Shouche, Y. S., Mourya, D. T., & Pant, U. (2002). New Helicoverpa armigera Hbn cell line from larval hemocyte for baculovirus studies. In Vitro Cell. Dev. Biol. Anim. 38:262-264; Sudeep, A. B., Mourya, D. T., & Mishra, A. C. (2005). Insect cell culture in research: Indian scenario. Indian Journal of Medical Research, 121(6), 725.
Notes
Morphology: Heterogeneous population of cells consists mainly of epithelial-like cells (70%), followed by fibroblast-like (27%), and multinucleated giant (3%) cells; Karyology: chromosome number ranged from 45 to 185; Growth characteristics: population-doubling time of approximately 60 h.
Medium
TNM-FH + 10% FBS
Cell Culture Collection
A B Sudeep, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, India.
Status
Microorganism Propagation
Able to replicate Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Spodoptera litura multiple nucleopolyhedrovirus (SfltMNPV), Helicoverpa armigera single nucleopolyhedrovirus (HaSNPV)
Adventitious Microorganism
Originally infected with microsporidium Nosema heliothids: using the antiprotozoan drug Metrogyl 400 and simultaneous heat treatment, the parasite was removed from the culture at passage 9.
