QAU-BTI-Tn9-4s
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
QAU-BTI-Tn9-4s
Accession No.
CVCL_AV37
Stage
Tissue
Initiator(s)
M. Shan
References
Meng M.-J., Li T.-L., Li C.-L., Li G.-X. (2008). A SUSPENDED CELL LINE FROM TRICHOPLUSIA NI (LEPIDOPTERA): CHARACTERIZATION AND EXPRESSION OF RECOMBINANT PROTEINS. Insect Science, 15, 423-428; Shan M., Zhang S.-Y., Jiang L., Ma M., Li G.-X. (2011). SUSCEPTIBILITY TO ACMNPV AND EXPRESSION OF RECOMBINANT PROTEINS BY A NOVEL CELL CLONE DERIVED FROM A TRICHOPLUSIA NI QAU-BTI-TN9-4S CELL LINE. Virologica Sinica 26:297-305(2011); Zheng G.-L., Zhou H.-X., Li C.-Y. (2014). SERUM-FREE CULTURE OF THE SUSPENSION CELL LINE QB-TN9-4S OF THE CABBAGE LOOPER, TRICHOPLUSIA NI, IS HIGHLY PRODUCTIVE FOR VIRUS REPLICATION AND RECOMBINANT PROTEIN EXPRESSION. Journal of Insect Science, 14, 24-24; Geisler C., Jarvis D.L. (2017). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein Expression and Purification, 144, 25-32.
Notes
Morphology: this line consists of two major morphological types, approximately 70% spindle-shaped cells (mean diameter = 18.22 × 57.70 μm) and 30% round cells (mean diameter = 22.19 μm); Growth characteristics: can be maintained as a suspension culture with no formation of cell aggregation or cell clumps, population doubling time = 25.4 hr; Unique properties: produces high levels of recombinant β-galactosidase and secreted alkaline phosphatase using a baculovirus expression vector system (3.97 ± 0.13 and × 104 IU/mL and 3.48 ± 0.40 IU/mL, respectively).
Medium
TNM-FH medium + 20% FBS
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
